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All the experiments were conducted in triplicate. The differences between the treatments were analyzed using one-way ANOVA followed by Tukey#x27;s multiple comparison test using GraphPad Prism 7.00 for Windows, GraphPad Software, La Jolla California USA . A avaliao das clulas infiltrantes do tumor foi feita por citometria de fluxo e os dados analisados no software GraphPad Prism. O tumor foi retirado e macerado, foi feita a contagem de clulas do tumor de cada animal e do total de clulas foram separadas 106 clulas que para serem submetidas fenotipagem por citometria utilizando anticorpos contra.
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